DNA

Part:BBa_K2908677:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-17)


GAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 439
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 134


Design Notes

We considered most about how to assemble to make it more effective as a transcription factor.


Source

We assembled Gal4(BBa_K105007) and VP16(BBa_K105001).

References